|LIFE (Laser Induced Fluorescent Emission) in the life science and material analysis
|Different tissues illuminated by laser, emit different color of light. This
color characteristics (fluorescent) is a power tool to identify different
types of biological tissues and its chemical compositions.
|Fiber optic catheter is inserted into the
coronary artery to detect the plaque which
causes the occlusion.
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Fluorescence is used in the life sciences generally as a
non-destructive way of tracking or analysis biological molecules by
means of fluorescence. Some protein or small molecules in cells are
naturally fluorescent, this is called intrinsic or autofluorescence (such
as NADH, tryptophan or endogenous Chlorophyll, Phycoerythrin or
green fluorescent protein), alternatively specific or general protein,
nucleic acids, lipids or small molecules can be "labelled" with an
extrinsic fluorophore, a fluorescent dye which can be a small molecule,
protein or quantum dot. Several techniques exist, often exploiting
additional properties, such as fluorescence resonance energy transfer,
where the energy is passed non-radiatively to a particular
neighbouring dye, allowing proximity to be detected; another is the
change in proprieties, such as intensity, of certain dyes depending on
their environment allowing their use in structural studies.
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